CARBON DIOXIDE AND ACETATE UTILIZATION BY CLOSTRIDIUM KLUYVERI
نویسندگان
چکیده
منابع مشابه
Carbon dioxide and acetate utilization by Clostridium kluyveri. II. Synthesis of amino acids.
The isolation and identification of labeled amino acids from the acid hydrolysates of cells of Clostridium kluyveri grown in synthetic medium containing ethanol, acetate, and CO* as carbon sources, and with Cl402 or acetate-l-Cl4 as labeled substrate, have been reported previously (28). Alanine, serine, glycine, threonine, and aspartic acid so obtained have been degraded to determine the positi...
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In 1952, Korkes (1) reported that cell-free extracts of Clostri&urn kluyveri were capable of reducing pyridine nucleotides with hydrogen. Subsequent investigations have shown that a heatstable cofactor of undetermined nature present in boiled cell extracts was required (2). Pyridine nucleotide reduction with hydrogen has since been demonstrated in a number of other microorganisms (3-5). The pre...
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Chloroplasts isolated by methods similar to that described by Arnon et al. (1) are capable of incorporating acetate carbon into both lipid and n)on-lipid materials when fortified with appropriate cofactors (2, 3,4, 5). The actual rate of incorporation of acetate carbon in these preparations was, however, very small compared with the normal rate of photosynthesis (1-2 jumoles/mg chlorophyll'hr) ...
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The experiments were done with dried cells of C. kluyveri, Lots Q and R, prepared by the method of Stadtman and Barker (2). Lot R contained 1.2 pM of volatile acids, consisting mostly of caproic acid with a little acetic and butyric acids, and 3.2 I.LM of non-protein amino nitrogen per 25 mg. of dried cells. The enzymes responsible for acetylation of amino acids are pot readily inactivated by e...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1954
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)65486-9